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7 March 2022 Organizing & interfacing multiphoton-printed biomaterials with model cellular systems
Jason B. Shear, Bryan Kaehr, Kyle Michelson, Swayamdipta Bhaduri, Basu Aryal, Xuening Zhou
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Proceedings Volume PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII; PC1196504 (2022) https://doi.org/10.1117/12.2610310
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Cellular studies using model in vitro systems limit environmental complexities that may obscure input-outcome relationships. To address the need for more relevant model systems, we have developed a platform for interfacing biomaterials with cultured cells with high spatiotemporal control. Pulsed near-infrared light is used to create protein-based cellular landscapes in a direct-write, multiphoton photo-crosslinking process patterned using a dynamic mask. High-resolution 3D environments are created either in advance of cellular application, or in the presence of viable cells, yielding dynamically controllable enclosures and surfaces for organizing cellular communities that more accurately reproduce mechanical, chemical, and convective properties of native environments.
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Jason B. Shear, Bryan Kaehr, Kyle Michelson, Swayamdipta Bhaduri, Basu Aryal, and Xuening Zhou "Organizing & interfacing multiphoton-printed biomaterials with model cellular systems", Proc. SPIE PC11965, Multiphoton Microscopy in the Biomedical Sciences XXII, PC1196504 (7 March 2022); https://doi.org/10.1117/12.2610310
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KEYWORDS
Systems modeling
Control systems
Digital Light Processing
Digital micromirror devices
In vitro testing
Modulation
Natural surfaces
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