Multi-window SRS imaging using a rapid widely tunable fiber laser

Hongli Ni; Peng Lin; Yifan Zhu; Meng Zhang; Yuying Tan; Yuewei Zhan; Ji-Xin Cheng

doi:10.1117/12.2606885

2 March 2022 Multi-window SRS imaging using a rapid widely tunable fiber laser

Hongli Ni, Peng Lin, Yifan Zhu, Meng Zhang, Yuying Tan, Yuewei Zhan, Ji-Xin Cheng

Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730C (2022) https://doi.org/10.1117/12.2606885
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Due to the limited spectral coverage of current SRS systems, most SRS applications utilized a narrow Raman band (<300 cm-1). Multi-window SRS imaging covering C-H, C-D and fingerprint regions offers richer chemical information. In this work, we present a multi-window SRS imaging system with a rapid widely tunable fiber laser. We implemented auto-balanced detection to enhance the signal-to-noise ratio of stimulated Raman loss by 23 times. We demonstrated SRS metabolic imaging of fungi, cancer cells, and Caenorhabditis elegans across the C-H, silent and fingerprint Raman windows. Our results showcase the potential of the multi-window SRS system for a broad range of applications.

Conference Presentation

Citation Download Citation

Hongli Ni, Peng Lin, Yifan Zhu, Meng Zhang, Yuying Tan, Yuewei Zhan, and Ji-Xin Cheng "Multi-window SRS imaging using a rapid widely tunable fiber laser", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730C (2 March 2022); https://doi.org/10.1117/12.2606885

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