Cell cycle analysis from phase and fluorescence images with a simple and compact microscope

Ondrej Mandula; Jean-Philippe Kleman; Francoise LACROIX; Cedric Allier; Lionel Herve; Sophie Morales

doi:10.1117/12.2636853

30 May 2022 Cell cycle analysis from phase and fluorescence images with a simple and compact microscope

Ondrej Mandula, Jean-Philippe Kleman, Francoise LACROIX, Cedric Allier, Lionel Herve, Sophie Morales

Proceedings Volume PC12136, Unconventional Optical Imaging III; PC1213610 (2022) https://doi.org/10.1117/12.2636853
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

We used a simple setup for phase and fluorescence time-lapse imaging of thousands of cells in parallel directly in the incubator. These images allow us to make a population based cell-cycle analysis comparable to flow-cytometry, but also give us an access to the analysis on the level of individual cells followed over several cell cycles. We can observe a thousand cells in parallel. We show a FUCCI marked HeLa cell culture observed over several days directly in the standard incubator. We compare our analysis to the flow cytometer data and show that we can produce a statistically relevant time-resolved measurement.

Conference Presentation

Citation Download Citation

Ondrej Mandula, Jean-Philippe Kleman, Francoise LACROIX, Cedric Allier, Lionel Herve, and Sophie Morales "Cell cycle analysis from phase and fluorescence images with a simple and compact microscope", Proc. SPIE PC12136, Unconventional Optical Imaging III, PC1213610 (30 May 2022); https://doi.org/10.1117/12.2636853

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