Spatio-temporal optical coherence tomography (STOC-T) focal plane adjustment in the mouse retina aided by a fundus camera

Wiktor Kulesza; Kamil Łuczkiewicz; Piotr Węgrzyn; Sławomir Tomczewski; Dawid Borycki; Piotr Ciąćka; Onur Cetinkaya; Maciej Wielgo; Katarzyna Kordecka; Anna Galińska; Egidijus Auksorius; Andrzej Foik; Robert J. Zawadzki; Maciej Wojtkowski; Andrea Curatolo

doi:10.1117/12.2652956

27 March 2023 Spatio-temporal optical coherence tomography (STOC-T) focal plane adjustment in the mouse retina aided by a fundus camera

Wiktor Kulesza, Kamil Łuczkiewicz, Piotr Węgrzyn, Sławomir Tomczewski, Dawid Borycki, Piotr Ciąćka, Onur Cetinkaya, Maciej Wielgo, Katarzyna Kordecka, Anna Galińska, Egidijus Auksorius, Andrzej Foik, Robert J. Zawadzki, Maciej Wojtkowski, Andrea Curatolo

Author Affiliations +

Proceedings Volume PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII; PC123671T (2023) https://doi.org/10.1117/12.2652956
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

We present a novel mouse eye imaging system based on the Spatio-Temporal Optical Coherence Tomography (STOC-T) technique capable of acquiring structural image of a mouse retina at a volumetric rate of 112 Hz. A fundus camera and white light illumination aid the alignment of the mouse and the adjustment of the focal plane in the mouse retina for the STOC-T image. The fundus camera is calibrated so that when the white-light image of the mouse eye fundus appears in focus after the appropriate gel thickness is selected for a given mouse and bi-concave lens, the corresponding near infrared STOC-T image of the photoreceptor layer is also in focus, albeit with minor shifts. We present images of retinal and choroidal tissue from a B6 albino wild type mouse after the focal plane adjustment with richness of details.

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Wiktor Kulesza, Kamil Łuczkiewicz, Piotr Węgrzyn, Sławomir Tomczewski, Dawid Borycki, Piotr Ciąćka, Onur Cetinkaya, Maciej Wielgo, Katarzyna Kordecka, Anna Galińska, Egidijus Auksorius, Andrzej Foik, Robert J. Zawadzki, Maciej Wojtkowski, and Andrea Curatolo "Spatio-temporal optical coherence tomography (STOC-T) focal plane adjustment in the mouse retina aided by a fundus camera", Proc. SPIE PC12367, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVII, PC123671T (27 March 2023); https://doi.org/10.1117/12.2652956

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