Needle-shaped beam optical-resolution photoacoustic microscopy with an extended depth of field

Rui Cao; Jingjing Zhao; Lei Li; Lin Du; Yide Zhang; Yilin Luo; Laiming Jiang; Samuel Davis; Qifa Zhou; Adam de la Zerda; Lihong Wang

doi:10.1117/12.2650561

9 March 2023 Needle-shaped beam optical-resolution photoacoustic microscopy with an extended depth of field

Rui Cao, Jingjing Zhao, Lei Li, Lin Du, Yide Zhang, Yilin Luo, Laiming Jiang, Samuel Davis, Qifa Zhou, Adam de la Zerda, Lihong Wang

Author Affiliations +

Proceedings Volume PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023; PC123790G (2023) https://doi.org/10.1117/12.2650561
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

To extend the depth of field (DOF) in optical-resolution photoacoustic microscopy (OR-PAM), we propose the needle-shaped beam photoacoustic microscopy (NB-PAM) via customized diffractive optical elements to extend the DOF, featuring a well-maintained beam diameter, a uniform axial intensity distribution, and negligible sidelobes. The advantage of using NB-PAM with an improved DOF has been demonstrated by both histology-like imaging of fresh slide-free organs using 266 nm laser and in vivo mouse brain vasculature imaging using 532 nm laser. Our approach provides new perspectives for slide-free intraoperative pathological imaging and various in vivo organ-level imaging applications.

Conference Presentation

Citation Download Citation

Rui Cao, Jingjing Zhao, Lei Li, Lin Du, Yide Zhang, Yilin Luo, Laiming Jiang, Samuel Davis, Qifa Zhou, Adam de la Zerda, and Lihong Wang "Needle-shaped beam optical-resolution photoacoustic microscopy with an extended depth of field", Proc. SPIE PC12379, Photons Plus Ultrasound: Imaging and Sensing 2023, PC123790G (9 March 2023); https://doi.org/10.1117/12.2650561

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