Endoscopic multiphoton fluorescence lifetime imaging of NADH (Conference Presentation)

Julius Heitz; Lukas Braun; Dennis Eggert; Hauke Studier; Wolfgang Becker; Christian Betz

doi:10.1117/12.2666398

15 March 2023 Endoscopic multiphoton fluorescence lifetime imaging of NADH (Conference Presentation)

Julius Heitz, Lukas Braun, Dennis Eggert, Hauke Studier, Wolfgang Becker, Christian Betz

Proceedings Volume PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; PC123840W (2023) https://doi.org/10.1117/12.2666398
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Clinical practitioners consider an abnormal cell metabolism as hallmark of carcinogenesis. Cellular energy metabolism is accessible by imaging of the fast autofluorescence decay of the endogenous fluorophore NADH. This technique is called metabolic fluorescence lifetime imaging (metabolic FLIM), best performed with multiphoton excitation and the rapid, precise and quantitative TCSPC technology from Becker&Hickl GmbH. However, conventional multiphoton FLIM microscopes rely on surface layer tissue access or excised tissue samples. Imaging inside the body for medical diagnostics is routinely accomplished with endoscopes. Here we present multiphoton metabolic FLIM of NADH performed through an endoscope, with significant applications in oncology and beyond.

Conference Presentation

Citation Download Citation

Julius Heitz, Lukas Braun, Dennis Eggert, Hauke Studier, Wolfgang Becker, and Christian Betz "Endoscopic multiphoton fluorescence lifetime imaging of NADH (Conference Presentation)", Proc. SPIE PC12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, PC123840W (15 March 2023); https://doi.org/10.1117/12.2666398

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KEYWORDS

Fluorescence lifetime imaging

Nicotinamide adenine dinucleotide

Endoscopy

Multiphoton fluorescence microscopy

Autofluorescence

Tissues

Endoscopes

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