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30 March 2023 Adaptive optics 3d structured illumination super resolution microscopy with fast remote focusing
Danail Stoychev, Richard Parton, Anarita Faria, Matthew Wincott, Ian M. Dobbie, Lothar Schermelleh, Ilan Davis, Martin J. Booth, Jingyu Wang
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Proceedings Volume PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; PC1238501 (2023) https://doi.org/10.1117/12.2650706
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Super-resolution microscopy allows the observation of sub-cellular structures with a resolution beyond diffraction limit of conventional fluorescence microscopy. However, most super-resolution microscopes have a limited imaging depth due to the inhomogeneous refractive index of the sample that leads to optical aberrations. Adaptive optics has been successfully adopted by many imaging techniques, including 3D Structured illumination microscopy (SIM). We use a fast deformable mirror to modulate the wavefront of fluorescence to compensate for optical aberration and changing focus position at the same time. Adaptive optics successfully extends the depth, the range and the speed of 3D-SIM imaging.
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Danail Stoychev, Richard Parton, Anarita Faria, Matthew Wincott, Ian M. Dobbie, Lothar Schermelleh, Ilan Davis, Martin J. Booth, and Jingyu Wang "Adaptive optics 3d structured illumination super resolution microscopy with fast remote focusing", Proc. SPIE PC12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, PC1238501 (30 March 2023); https://doi.org/10.1117/12.2650706
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KEYWORDS
Adaptive optics
Super resolution microscopy
Microscopes
3D image processing
Super resolution
Imaging systems
Luminescence
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