Optical gearbox enabled high-speed laser scanning multiphoton microscopy (Conference Presentation)

Jianian Lin; Zongyue Cheng; Meng Cui

doi:10.1117/12.2650074

16 March 2023 Optical gearbox enabled high-speed laser scanning multiphoton microscopy (Conference Presentation)

Jianian Lin, Zongyue Cheng, Meng Cui

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC1239008 (2023) https://doi.org/10.1117/12.2650074
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Laser scanning multiphoton microscopy is widely used for in vivo imaging applications. Commonly employed systems can offer an imaging rate of tens of Hz. With the emergence of high-speed function indicators, an imaging rate at hundreds of Hz is required. Recently, we developed the optical gearbox, which can convert commonly employed imaging systems and sources for high-speed imaging with a frame rate ranging from tens of Hz to 1,000 Hz. Here, we present our latest work which showcases the performance of optical gearbox based two-photon laser scanning microscopy for in vivo imaging of neuronal and vascular dynamics in mouse brain.

Conference Presentation

Citation Download Citation

Jianian Lin, Zongyue Cheng, and Meng Cui "Optical gearbox enabled high-speed laser scanning multiphoton microscopy (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239008 (16 March 2023); https://doi.org/10.1117/12.2650074

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available