High-throughput tomographic fluorescence imaging with a synchronized camera array (Conference Presentation)

Kevin C. Zhou; Joakim Jönsson; Clare Cook; Kyung Chul Lee; Xi Yang; Shiqi Xu; Jennifer Bagwell; Archan Chakraborty; Donald Fox; Michel Bagnat; Roarke Horstmeyer

doi:10.1117/12.2649458

16 March 2023 High-throughput tomographic fluorescence imaging with a synchronized camera array (Conference Presentation)

Kevin C. Zhou, Joakim Jönsson, Clare Cook, Kyung Chul Lee, Xi Yang, Shiqi Xu, Jennifer Bagwell, Archan Chakraborty, Donald Fox, Michel Bagnat, Roarke Horstmeyer

Author Affiliations +

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900C (2023) https://doi.org/10.1117/12.2649458
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We present a high-throughput computational imaging system capable of performing dense, volumetric fluorescence imaging of freely moving organisms at up to 120 volumes per second. Our method, termed 2pi Fourier light field tomography (2pi-FLIFT), consists of a planar array of 54 cameras and a parabolic mirror serving as the primary objective that allows for synchronized multi-view video capture over ~2pi steradians. 2pi-FLIFT features a novel 3D reconstruction algorithm that recovers both the 3D fluorescence distribution and attenuation map of dynamic samples. We demonstrate 2pi-FLIFT on important, freely moving model organisms, such as zebrafish and fruit fly larvae.

Conference Presentation

Citation Download Citation

Kevin C. Zhou, Joakim Jönsson, Clare Cook, Kyung Chul Lee, Xi Yang, Shiqi Xu, Jennifer Bagwell, Archan Chakraborty, Donald Fox, Michel Bagnat, and Roarke Horstmeyer "High-throughput tomographic fluorescence imaging with a synchronized camera array (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900C (16 March 2023); https://doi.org/10.1117/12.2649458

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