Automated platform for dual-color 3D imaging of single cells at high resolution and throughput (Conference Presentation)

Petra Paiè; Andrea Comi; Alessia Candeo; Gianmaria Calisesi; Federico Sala; Francesco Ceccarelli; Korbinian Muhlberger; Michael Fokine; Mark Neil; Gianluca Valentini; Roberto Osellame; Andrea Bassi; Francesca Bragheri

doi:10.1117/12.2650263

16 March 2023 Automated platform for dual-color 3D imaging of single cells at high resolution and throughput (Conference Presentation)

Petra Paiè, Andrea Comi, Alessia Candeo, Gianmaria Calisesi, Federico Sala, Francesco Ceccarelli, Korbinian Muhlberger, Michael Fokine, Mark Neil, Gianluca Valentini, Roberto Osellame, Andrea Bassi, Francesca Bragheri

Author Affiliations +

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC123900O (2023) https://doi.org/10.1117/12.2650263
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Heterogeneity plays an important role in medicine and biology, which can be investigated by exploiting single cell analysis (SCA). Among SCA methods, imaging cytometry allows the analysis of individual 2D and 3D spatial features. Here we present a femtosecond laser fabricated optofluidic automated platform encompassing a thermo-optic phase shifter, cylindrical lenses and a microfluidic network to generate and shift a dual-color patterned light sheet within a microchannel where the samples of interest flow. The device can be used as add-on and can provide an acquisition rate of about 1 cell/second, or subnuclear resolution at the single cell level.

Conference Presentation

Citation Download Citation

Petra Paiè, Andrea Comi, Alessia Candeo, Gianmaria Calisesi, Federico Sala, Francesco Ceccarelli, Korbinian Muhlberger, Michael Fokine, Mark Neil, Gianluca Valentini, Roberto Osellame, Andrea Bassi, and Francesca Bragheri "Automated platform for dual-color 3D imaging of single cells at high resolution and throughput (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900O (16 March 2023); https://doi.org/10.1117/12.2650263

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