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OCT has been widely employed for capturing dynamic processes in biological tissues via (2+1)D imaging, but are currently limited in (3+1)D imaging by both scan time and storage efficiency. Temporal non-uniform compressive sampling has been previously demonstrated on both simulations and existing data with accurate reconstruction of intracellular motility with up to 8-fold compression. Here we present the experimental implementation of this technique on a spectral-domain OCT system, with demonstration of significantly reduced memory overhead and total imaging time at 4- and 8-fold compression, which enables new applications in high-throughput assays employing (3+1)D imaging.
Lin Yang,Pan Ji, andAmy Oldenburg
"High-throughput 4D OCT of mammary spheroids with temporal non-uniform compressive sampling (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900U (16 March 2023); https://doi.org/10.1117/12.2649206
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Lin Yang, Pan Ji, Amy Oldenburg, "High-throughput 4D OCT of mammary spheroids with temporal non-uniform compressive sampling (Conference Presentation)," Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC123900U (16 March 2023); https://doi.org/10.1117/12.2649206