Evaluation of the preservation of tissue metabolic function in fresh and fixed epithelium using label-free two-photon fluorescence microscopy (Conference Presentation)

Adriana Sánchez-Hernández; Christopher Polleys; Irene Georgakoudi

doi:10.1117/12.2650776

16 March 2023 Evaluation of the preservation of tissue metabolic function in fresh and fixed epithelium using label-free two-photon fluorescence microscopy (Conference Presentation)

Adriana Sánchez-Hernández, Christopher Polleys, Irene Georgakoudi

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239108 (2023) https://doi.org/10.1117/12.2650776
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Two-photon excited fluorescence (TPEF) can provide metabolic information of the cellular microenvironment. Metabolic changes are intimately associated with numerous diseases. It has been suggested that the metabolic interpretations from fresh tissue imaging can be maintained in tissue samples post-fixation. Given the large availability of fixed tissues for various conditions, we sought to evaluate the impact of fixation on several TPEF-extracted metabolic indicators of unstained tissues based on endogenous intensity and lifetime TPEF measurements. Our results indicate that the metabolic interpretations of fixed tissue cannot be used as a representation of the in vivo environment or that of freshly excised specimens.

Conference Presentation

Citation Download Citation

Adriana Sánchez-Hernández, Christopher Polleys, and Irene Georgakoudi "Evaluation of the preservation of tissue metabolic function in fresh and fixed epithelium using label-free two-photon fluorescence microscopy (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239108 (16 March 2023); https://doi.org/10.1117/12.2650776

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Tissues

Two photon excitation microscopy

Tissue optics

Biological research

Luminescence

Data analysis

Data processing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years