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5 October 2023 Bacterial biofilm formation with optical tweezers
Anna S. Bezryadina, Czarlyn Camba, Brooke Walter-Lakes
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Proceedings Volume PC12663, Molecular and Nanophotonic Machines VI; PC1266306 (2023) https://doi.org/10.1117/12.2676168
Event: SPIE Organic Photonics + Electronics, 2023, San Diego, California, United States
Abstract
Biofilm is produced when a bacteria’s environment becomes hostile and uses biofilm as protection from the environment. In this work, we investigate the biofilm formation of Bacillus subtilis bacteria within minimal biofilm-promoting media (MSgg media) and how optical trapping affects bacteria aggregation and biofilm development. In low-nutrition media, B. subtilis secretes a glue-like substance and ultimately forms a biofilm. We use an optical tweezers system to observe bacteria division, reorganization, aggregation, and clustering with and without optical trapping. The study of optically controlled biofilm formation enables us to create novel models for inducing and suppressing biofilm development with lasers.
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Anna S. Bezryadina, Czarlyn Camba, and Brooke Walter-Lakes "Bacterial biofilm formation with optical tweezers", Proc. SPIE PC12663, Molecular and Nanophotonic Machines VI, PC1266306 (5 October 2023); https://doi.org/10.1117/12.2676168
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KEYWORDS
Biofilms
Bacteria
Optical tweezers
Laser development
Antimicrobial agents
Blue lasers
Glasses
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