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13 March 2024 Adaptive line-illumination scheme for high-speed two-photon fluorescence microscopy
Yunyang Li, Shu Guo, Weijian Yang
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Proceedings Volume PC12828, Neural Imaging and Sensing 2024; PC1282805 (2024) https://doi.org/10.1117/12.3003342
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity in cellular resolution. Our microscope uses line illumination with an adaptive sampling scheme. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the tissue, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies), and samples a large area of them in a single measurement. This significantly increases the imaging speed and reduces the overall laser power on the sample. We characterized the imaging resolution and verified the concept of adaptive sampling through phantom samples. Our approach holds great promise for high-throughput neural activity imaging.
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Yunyang Li, Shu Guo, and Weijian Yang "Adaptive line-illumination scheme for high-speed two-photon fluorescence microscopy", Proc. SPIE PC12828, Neural Imaging and Sensing 2024, PC1282805 (13 March 2024); https://doi.org/10.1117/12.3003342
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KEYWORDS
Biological samples
Two photon excitation microscopy
Nervous system
Brain
Digital micromirror devices
High speed imaging
Light sources and illumination
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