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13 March 2024 Development of a label-free optofluidic platform based on quantitative-phase digital holographic microscopy and microfluidics to perform biophysical phenotyping of human cells
Erik Bélanger, Gabrielle Jess, Céline Larivière-Loiselle, Sara Mattar, Niraj Patel, Zahra Yazdani-Najafabadi, Mohamed Haouat, Johan Chaniot, Émile Rioux-Pellerin, Marie-Ève Crochetière, Jean-Xavier Giroux, Antoine Allard, Patrick Desrosiers, Pierre Marquet
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Proceedings Volume PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII; PC128370D (2024) https://doi.org/10.1117/12.3002808
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Progress achieved in the field of stem-cell technology allows the reprogramming of patient-derived cells, obtained from urine or skin biopsies, into induced pluripotent stem cells that can then be differentiated into any cell types. Within this framework, techniques, being able to accurately and non-invasively characterize cell structure, morphology, and dynamics, represent very promising approaches to identify disease-specific cell phenotypes. Consequently, we will present how a label-free optofluidic platform, based on quantitative-phase digital holographic microscopy along with various experimental developments in microfluidics, constitutes a very appealing cell imaging methodology to identify, through the measurement of biophysical properties, specific cell phenotypes.
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Erik Bélanger, Gabrielle Jess, Céline Larivière-Loiselle, Sara Mattar, Niraj Patel, Zahra Yazdani-Najafabadi, Mohamed Haouat, Johan Chaniot, Émile Rioux-Pellerin, Marie-Ève Crochetière, Jean-Xavier Giroux, Antoine Allard, Patrick Desrosiers, and Pierre Marquet "Development of a label-free optofluidic platform based on quantitative-phase digital holographic microscopy and microfluidics to perform biophysical phenotyping of human cells", Proc. SPIE PC12837, Microfluidics, BioMEMS, and Medical Microsystems XXII, PC128370D (13 March 2024); https://doi.org/10.1117/12.3002808
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KEYWORDS
Digital holography
Microfluidics
Cell phenotyping
Holography
Microscopy
Optofluidics
Imaging devices
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