Capillary blood flow imaging in awake mice using dynamic light scattering OCT and two photon microscopy

Sida Liang; Qi Pian; Mohammed Alfadhel; Buyin Fu; Jianbo Tang; Sava Sakadzic

doi:10.1117/12.3023450

13 March 2024 Capillary blood flow imaging in awake mice using dynamic light scattering OCT and two photon microscopy

Sida Liang, Qi Pian, Mohammed Alfadhel, Buyin Fu, Jianbo Tang, Sava Sakadzic

Proceedings Volume PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI; PC128410F (2024) https://doi.org/10.1117/12.3023450
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Accurate assessment of cerebral microvascular flow is crucial for understanding brain functioning and neurovascular diseases. Dynamic Light Scattering Optical Coherence Tomography (DLS-OCT) has been used to obtain blood velocity measurements in a large number of microvascular segments, including arterioles, capillaries, and venules in anesthetized mice. However, anesthesia induces large changes in the microvascular blood flow. Imaging awake animals by DLS-OCT is preferable, but very challenging due to motion artifacts. Here, we present the first DLS-OCT measurements of cortical microvascular blood flow in awake mice, made possible by an innovative algorithm based on Vertical Displacement at Inflection (IVD) in velocity distribution.

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Sida Liang, Qi Pian, Mohammed Alfadhel, Buyin Fu, Jianbo Tang, and Sava Sakadzic "Capillary blood flow imaging in awake mice using dynamic light scattering OCT and two photon microscopy", Proc. SPIE PC12841, Dynamics and Fluctuations in Biomedical Photonics XXI, PC128410F (13 March 2024); https://doi.org/10.1117/12.3023450

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KEYWORDS

Blood circulation

Capillaries

Biological imaging

Dynamic light scattering

Optical coherence tomography

Two photon excitation microscopy

Animal model studies

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