3D optoacoustic mesoscopy of neuromelanin in entire human midbrain organoids

Ludwig Englert; María Lacalle-Aurioles; Nguyen-Vi Mohamed; Paula Lépine; Meghna Mathur; Vasilis Ntziachristos; Thomas M. Durcan; Juan Aguirre

doi:10.1117/12.3001325

13 March 2024 3D optoacoustic mesoscopy of neuromelanin in entire human midbrain organoids

Ludwig Englert, María Lacalle-Aurioles, Nguyen-Vi Mohamed, Paula Lépine, Meghna Mathur, Vasilis Ntziachristos, Thomas M. Durcan, Juan Aguirre

Author Affiliations +

Proceedings Volume PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024; PC128421T (2024) https://doi.org/10.1117/12.3001325
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

Optical imaging of human midbrain organoids in the field of Parkinson research is challenging using current established technologies since they require lengthy and invasive clearing methods, which preclude live organoid observations and longitudinal studies.

Raster-Scanning Optoacoustic Mesoscopy (RSOM) overcomes this limitation by utilizing the intrinsic optical absorption contrast based on the optoacoustic effect. We show that RSOM is able to provide fast quantitative visualization of the neuromelanin content in intact large organoids at single cell resolution.

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Ludwig Englert, María Lacalle-Aurioles, Nguyen-Vi Mohamed, Paula Lépine, Meghna Mathur, Vasilis Ntziachristos, Thomas M. Durcan, and Juan Aguirre "3D optoacoustic mesoscopy of neuromelanin in entire human midbrain organoids", Proc. SPIE PC12842, Photons Plus Ultrasound: Imaging and Sensing 2024, PC128421T (13 March 2024); https://doi.org/10.1117/12.3001325

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