Quantitative imaging of murine organs with deep ultraviolet fluorescence microscopy

Ko Hui Tan; Alexander Si Kai Yong; Joel Ang; Kaicheng Liang

doi:10.1117/12.3002459

13 March 2024 Quantitative imaging of murine organs with deep ultraviolet fluorescence microscopy

Ko Hui Tan, Alexander Si Kai Yong, Joel Ang, Kaicheng Liang

Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC1284601 (2024) https://doi.org/10.1117/12.3002459
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Quantitative fluorescence imaging of entire organs at the cellular level is crucial in understanding tissue structure and function. However, it is often time-consuming and labor-intensive to section tissue for high-resolution microscopy. We developed a deep ultraviolet fluorescence microscope for rapid surface imaging of thick unsectioned tissue at cellular resolution. Fast staining and tissue microscopy protocols were designed for practical use cases. We investigated the effects of vancomycin on mouse kidneys and also conducted studies on uterine ageing and its association with extracellular matrix protein expression. Deep ultraviolet fluorescence microscopy offers a promising approach in quantitative mapping of whole organs.

Conference Presentation

Citation Download Citation

Ko Hui Tan, Alexander Si Kai Yong, Joel Ang, and Kaicheng Liang "Quantitative imaging of murine organs with deep ultraviolet fluorescence microscopy", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC1284601 (13 March 2024); https://doi.org/10.1117/12.3002459

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