High-resolution virtual biopsy: non-invasive pathology using deep learning and optical coherence tomography

Aidan Van Vleck; Yonatan Winetraub; Jingjing Zhao; Adam de la Zerda

doi:10.1117/12.3004227

13 March 2024 High-resolution virtual biopsy: non-invasive pathology using deep learning and optical coherence tomography

Aidan Van Vleck, Yonatan Winetraub, Jingjing Zhao, Adam de la Zerda

Author Affiliations +

Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC128460M (2024) https://doi.org/10.1117/12.3004227
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Virtual biopsy enables non-invasive diagnosis through machine-learning analysis of high-resolution images. But difficulties in generating accurate co-registered training sets and the resolution/field-of-view (FOV) tradeoff have hindered clinical applications. We present a method enabling reliable and accurate co-registration of 3D cellular-resolution OCT of fresh human skin with downstream histology. Orientation data is encoded across the sample by photobleaching a fiduciary grid pattern into fluorescent gel encasing the tissue. These markers persist in histology sections, permitting accurate co-registration to the 3D volume within ~20µm, and enabling cellular-resolution imaging with a cm-level FOV by laterally tiling OCT volumes, crucial steps toward in-vivo high-resolution Virtual Biopsy.

Conference Presentation

Citation Download Citation

Aidan Van Vleck, Yonatan Winetraub, Jingjing Zhao, and Adam de la Zerda "High-resolution virtual biopsy: non-invasive pathology using deep learning and optical coherence tomography", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC128460M (13 March 2024); https://doi.org/10.1117/12.3004227

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Biopsy

Tissues

Optical coherence tomography

3D scanning

Image quality

Pathology

Education and training

Show All Keywords

Keywords/Phrases

Search In:

Publication Years