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13 March 2024 Small SPAD-arrays for confocal fluoresence lifetime imaging
Felix Koberling, Max Tillmann, Tino Roehlicke, Michael Wahl, Rainer Erdmann, Ivan Michel Antolovic
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Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470Y (2024) https://doi.org/10.1117/12.3001460
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Confocal microscopy is an essential tool in many academic disciplines due to its intrinsic sectioning capability. The combination with time-resolved single photon detectors and time-correlated single photon counting (TCSPC) devices has established it as the leading platform for time resolved investigation methods such as fluorescence lifetime imaging (FLIM). Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. In this work we present the two central hardware building blocks: PicoQuant’s latest multi-channel TCSPC device and a cooled high-performance 23-pixel SPAD-array developed jointly with Pi Imaging Technologies. We discuss how these open up new possibilities in time-resolved confocal microscopy.
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Felix Koberling, Max Tillmann, Tino Roehlicke, Michael Wahl, Rainer Erdmann, and Ivan Michel Antolovic "Small SPAD-arrays for confocal fluoresence lifetime imaging", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470Y (13 March 2024); https://doi.org/10.1117/12.3001460
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KEYWORDS
Confocal microscopy
Time correlated single photon counting
Fluorescence correlation spectroscopy
Fluorescence lifetime imaging
Data processing
Imaging technologies
Single photon detectors
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