A novel integration of 1P confocal- and multiphoton-FLIM in one system

Taravat Saeb Gilani; Lukas Braun; Julius Heitz; Hauke Studier; Wolfgang Becker

doi:10.1117/12.3005810

13 March 2024 A novel integration of 1P confocal- and multiphoton-FLIM in one system

Taravat Saeb Gilani, Lukas Braun, Julius Heitz, Hauke Studier, Wolfgang Becker

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC1284711 (2024) https://doi.org/10.1117/12.3005810
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

A novel imaging system is presented, bridging 1P confocal- and MP-FLIM in one measurement setup, for a non-invasive, early and accurate detection of tumor tissues. Integrating a unit consisting of an acousto-optical modulator (AOM) and a second harmonic generator (SHG), together with a tunable Ti:Sa laser, provides stabilized femtosecond pulses from 340 nm to 1300 nm with high pointing precision for microscopy, for the first time. This facilitates high precision metabolic imaging of individual cells through 1P FLIM, and deep tissue imaging through MP-FLIM. While SHG reveales insights into non-centrosymmetric structures in tissues, simultaneously. The system offers 2 confocal FLIM detection channels, 2 non-descanned detection channels for MP FLIM and 1 detection channel for SHG light. This talk emphasizes the instrumentation of the system, followed by presenting obtained metabolic images and optimized analysis of data images.

Conference Presentation

Citation Download Citation

Taravat Saeb Gilani, Lukas Braun, Julius Heitz, Hauke Studier, and Wolfgang Becker "A novel integration of 1P confocal- and multiphoton-FLIM in one system", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC1284711 (13 March 2024); https://doi.org/10.1117/12.3005810

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