Volumetric super-resolution imaging of tissues using a confocal pinhole array

Ben E. Urban Jr.; Abmael Oliveira; Thomas Livecchi; Hrebesh Subhash

doi:10.1117/12.3001027

13 March 2024 Volumetric super-resolution imaging of tissues using a confocal pinhole array

Ben E. Urban Jr., Abmael Oliveira, Thomas Livecchi, Hrebesh Subhash

Proceedings Volume PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII; PC1284905 (2024) https://doi.org/10.1117/12.3001027
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We present a novel technique for volumetric super-resolution imaging. Our technique, which is based on the principles of single molecule localization microscopy, utilizes a mirror cavity with a series of pinholes on one of the mirrors allowing for simultaneous optical sectioning of different imaging planes. In addition, we employ a unique machine learned algorithm for 3D localization of events that occur between different imaging planes. Our technique enables high-resolution imaging of thicker volumes than what is currently available using other single molecule localization techniques.

Conference Presentation

Citation Download Citation

Ben E. Urban Jr., Abmael Oliveira, Thomas Livecchi, and Hrebesh Subhash "Volumetric super-resolution imaging of tissues using a confocal pinhole array", Proc. SPIE PC12849, Single Molecule Spectroscopy and Superresolution Imaging XVII, PC1284905 (13 March 2024); https://doi.org/10.1117/12.3001027

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