Metasurface-enhanced mid-infrared microscopy for vibrational imaging in fixed and living cells

Steven H. Huang; Po-Ting Shen; Giovanni Sartorello; Aditya Mahalanabish; Gennady Shvets

doi:10.1117/12.3021441

13 March 2024 Metasurface-enhanced mid-infrared microscopy for vibrational imaging in fixed and living cells

Steven H. Huang, Po-Ting Shen, Giovanni Sartorello, Aditya Mahalanabish, Gennady Shvets

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC1285503 (2024) https://doi.org/10.1117/12.3021441
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We developed metasurface-enhanced mid-infrared microscopy for living cells, where cells are cultured on a metasurface-bottomed microwell and are probed from the bottom using a confocal laser scanning microscope with a quantum cascade laser (QCL) as the source. The mid-infrared light interacts with the cells through the near-field of the plasmonic nanoantennas in the metasurface, and the reflected light is detected in the far field. Using this setup, we imaged fixed cells at different vibrational bands for the chemical imaging of different biomolecules, including proteins, lipids, and nucleic acids. Additionally, we have monitored the real-time adhesion and spreading of living cells on the metasurface with protein contrast.

Conference Presentation

Citation Download Citation

Steven H. Huang, Po-Ting Shen, Giovanni Sartorello, Aditya Mahalanabish, and Gennady Shvets "Metasurface-enhanced mid-infrared microscopy for vibrational imaging in fixed and living cells", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC1285503 (13 March 2024); https://doi.org/10.1117/12.3021441

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