Measuring chemical changes during aging in vivo with label-free stimulated Raman scattering microscopy

Bryce Manifold; Bowen Yang; Denis Titov; Aaron Streets

doi:10.1117/12.3003529

13 March 2024 Measuring chemical changes during aging in vivo with label-free stimulated Raman scattering microscopy

Bryce Manifold, Bowen Yang, Denis Titov, Aaron Streets

Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550C (2024) https://doi.org/10.1117/12.3003529
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Aging related biological mechanisms are often difficult to probe in situ without exogenous fluorophores. Here, we leverage label-free stimulated Raman scattering (SRS) microscopy to provide new insights into aging in C. elegans. We demonstrate multispectral SRS imaging of whole worms in vivo with quantitative chemical insights across different ages. We show that both lipid and protein synthesis and compartmentalization are associated with aging in worms. Moreover, we observe notable SRS image differences when worms are subjected to calorie restriction, suggesting a promising avenue towards understanding calorie restriction’s enhancing effects on longevity when coupled to proteomic and metabolomic analysis.

Conference Presentation

Citation Download Citation

Bryce Manifold, Bowen Yang, Denis Titov, and Aaron Streets "Measuring chemical changes during aging in vivo with label-free stimulated Raman scattering microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550C (13 March 2024); https://doi.org/10.1117/12.3003529

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