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13 March 2024 Broadband chemically-specific imaging by synchrotron photothermal mid-IR microscopy
Aleksandr Razumtcev, Hans Bechtel, Garth J. Simpson
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Proceedings Volume PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024; PC128550Y (2024) https://doi.org/10.1117/12.3002072
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Broadband photothermal imaging using synchrotron radiation of the Lawrence Berkeley National Laboratory Advanced Light Source is demonstrated. Synchrotron-based photothermal spectroscopy simultaneously provides a significantly improved bandwidth over commercial laser-based approaches and enables sub-micron chemical imaging with at least a tenfold resolution improvement over FTIR microscopy. Synchrotron PTIR is shown to enable studying low-frequency aromatic bending modes of polymer samples. Furthermore, fluorescence detection of the photothermal effect was employed to demonstrate cell-specific broadband infrared imaging in brain tissues using nucleus-specific fluorescence dyes.
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Aleksandr Razumtcev, Hans Bechtel, and Garth J. Simpson "Broadband chemically-specific imaging by synchrotron photothermal mid-IR microscopy", Proc. SPIE PC12855, Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, PC128550Y (13 March 2024); https://doi.org/10.1117/12.3002072
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KEYWORDS
Biological imaging
Synchrotrons
Imaging spectroscopy
Infrared imaging
Mid-IR
Fourier transforms
Microscopy
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