Safety and Cleanliness Considerations

doi:10.1117/3.660403.ch15

Book: Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of Live Cell Imaging

Author(s): Barry R. Masters

Published: 2006

https://doi.org/10.1117/3.660403.ch15

Abstract

Two other topics that should not be overlooked are laser safety and caring for the optics in your microscope. Here is a summary of cautions to take when using lasers and light microscopes. 15.1 Laser Safety â¢ Read one or more source books on laser safety. â¢ Be aware of the different types of lasers, their damage thresholds, and the risks of ocular damage, skin damage, and fire associated with each type of laser. â¢ Be aware of the advantages (ocular protection) and disadvantages (no knowledge of position of the laser beam) of laser safety goggles. â¢ When designing a laser system on an optical table be sure that the laser beam is not at eye height. â¢ Be aware of the fire danger with lasers. Most materials in the clinic are flammable. 15.2 How to Clean Optics â¢ Optics should be cleaned at the following times: when there is a power loss; at regular maintenance intervals; when a laser cavity is opened; when a new set of mirrors is installed; when new optics are received. â¢ Learn how to correctly clean a microscope objective prior to and after use. â¢ Particles of dust can severely degrade the image quality of optical components. â¢ Improper cleaning of a microscope objective will cause microscopic scratches that will permanently degrade the image quality. Never use anything but lens-cleaning paper with spectroscopic-grade acetone and methanol. Note that these solvents can solubilize oil from your skin and transfer it to the surface of the optical element. â¢ Use a dust cover made from a conductive material to shield the microscope from dust.