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1 July 2008 Errata: Energy migration alters the fluorescence lifetime of Cerulean: implications for fluorescence lifetime imaging Forster resonance energy transfer measurements
Srinagesh V. Koushik, Steven S. Vogel
Author Affiliations +

Due to a publisher oversight, some corrections to the text and equations were not incorporated in this paper [ J. Biomed. Opt. 13, 031204 (2008)] prior to its publication. The online paper was replaced on 8 August 2008 by the correct version in its entirety. A listing of the most significant corrections follows here:

  • (1) The last sentence of the abstract was corrected to “We find a change in the fluorescent lifetime of Cerulean that correlates with energy migration and can result in significant errors when using Cerulean as a donor to measure FRET efficiencies based on fluorescence lifetimes.”

  • (2) The first sentence of the first paragraph on page 2 was corrected to “Recently fluorescence anisotropy has been used to reveal energy migration between two CFP molecules tethered together by a short poly-peptide linker.”

  • (3) In line 2 of Sec. 2.2, “22Venus” was changed to “Venus.”

  • (4) Equation 2 was corrected to:

    Eq. 2

    r=(IGII+2GI)

  • (5) Equation 3 was corrected to:

    Eq. 3

    I=I+2×G×I

  • (6) In line 19 of the second paragraph in the second column of page 4, “C5A” was corrected to “C5V.”

©(2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Srinagesh V. Koushik and Steven S. Vogel "Errata: Energy migration alters the fluorescence lifetime of Cerulean: implications for fluorescence lifetime imaging Forster resonance energy transfer measurements," Journal of Biomedical Optics 13(4), 049801 (1 July 2008). https://doi.org/10.1117/1.2979676
Published: 1 July 2008
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