Hybrid imaging of fluorescently labeled cancer drugs and label-free four-wave mixing microscopy of cancer cells and tissues

Rick Krabbendam; Martin Pool; Liesbeth G. de Vries; Herman L. Offerhaus; Jennifer L. Herek; Cees Otto

doi:10.1117/1.JBO.20.8.086006

13 August 2015 Hybrid imaging of fluorescently labeled cancer drugs and label-free four-wave mixing microscopy of cancer cells and tissues

Rick Krabbendam, Martin Pool, Liesbeth G. de Vries, Herman L. Offerhaus, Jennifer L. Herek, Cees Otto

Author Affiliations +

Journal of Biomedical Optics, Vol. 20, Issue 8, 086006 (August 2015). https://doi.org/10.1117/1.JBO.20.8.086006

Abstract

Fluorescent labels are well suited as tracers for cancer drug monitoring. Identifying cellular target regions of these drugs with a high resolution is important to assess the working principle of a drug. We investigate the applications of label-free nonresonant four-wave mixing (NR-FWM) microscopy in biological imaging in combination with fluorescence imaging of fluorescently labeled cancer drugs. Results from human A431 tumor cells with stained nuclei and incubated with IRdye 800CW labeled cancer drug cetuximab targeting epidermal growth factor receptor at the cell membrane show that NR-FWM is well suited for cellular imaging. A comparison of vibrationally nonresonant FWM imaging with vibrational resonant coherent anti-Stokes Raman scattering signals revealed nearly identical qualitative information in cellular imaging. NR-FWM is also suitable for tumor tissue imaging in combination with fluorescence imaging of IRdye 800CW labeled, human epidermal growth factor 2 targeting cancer drug pertuzumab and provides additional information over transmission microscopy.

Citation Download Citation

Rick Krabbendam, Martin Pool, Liesbeth G. de Vries, Herman L. Offerhaus, Jennifer L. Herek, and Cees Otto "Hybrid imaging of fluorescently labeled cancer drugs and label-free four-wave mixing microscopy of cancer cells and tissues," Journal of Biomedical Optics 20(8), 086006 (13 August 2015). https://doi.org/10.1117/1.JBO.20.8.086006

Published: 13 August 2015

Access the abstract

JOURNAL ARTICLE
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY