The commercial development of uncooled-microbolometer, long-wave infrared (LWIR) imagers, combined with advanced radiometric calibration methods developed at Montana State University, has led to new uses of thermal imagery in remote sensing applications. One specific novel use of these calibrated imagers is imaging of vegetation for CO 2 gas leak detection. During a four-week period in the summer of 2011, a CO 2 leak was simulated in a test field run by the Zero Emissions Research and Technology Center in Bozeman, Montana. An LWIR imager was deployed on a scaffold before and during the CO 2 release, viewing a vegetation test area that included regions of high and low CO 2 flux. Increased root-level CO 2 concentration caused plant stress that led to reduced thermal regulation of the vegetation, which was consistent with increased diurnal variation of IR emission observed in this study. In a linear regression, the IR data were found to have a strong relationship to the CO 2 emission and to be consistent with the location of leaking CO 2 gas. Reducing the continuous data set to one image per day weakened the regression fit, but maintained sufficient significance to indicate that this method could be implemented with once-daily airborne images.