The Moderate Resolution Imaging Spectroradiometer (MODIS), launched on the Terra and Aqua spacecrafts, was designed to collect complementary and comprehensive measurements of the Earth’s properties on a global scale. The 20 reflective solar bands (RSBs), covering a wavelength range from 0.41 to 2.1 μm, are calibrated on-orbit using regularly scheduled solar diffuser (SD) observations. Although primarily used for on-orbit gain derivation, the SD observations also facilitate the characterization of the detector signal-to-noise ratio (SNR). In addition to the calibration requirement of 2% for the reflectance factors and 5% for the radiances, the required SNRs are also specified for all RSB at their typical scene radiances. A methodology to characterize the on-orbit SNR for the MODIS RSB is presented. Overall performance shows that a majority of the RSB continue to meet the specification, therefore performing well. A temporal decrease in the SNR, observed in the short-wavelength bands, is attributed primarily to the decrease in their detector responses. With the exception of the inoperable and noisy detectors in band 6 identified prelaunch, the detectors of Aqua MODIS RSB perform better than Terra MODIS. The approach formulated for on-orbit SNR characterization can also be used by other sensors that use on-board SDs for their on-orbit calibration (e.g., Suomi National Polar-Orbiting Partnership [SNPP]-Visible Infrared Imaging Radiometer Suite).