2 April 1996 Cell damage by UVA radiation of a mercury microscopy lamp probed by autofluorescence modifications, cloning assay, and comet assay
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J. of Biomedical Optics, 1(2), (1996). doi:10.1117/12.233373
Abstract
Cell damage by low-power 365-nm radiation of a 50-W high-pressure mercury microscopy lamp was studied. Exposure of Chinese hamster ovary (CHO) cells to ultraviolet-A (UVA) radiation .10 kJ/m2 resulted in significant modifications of nicotinamide adenine dinucleotide (NADH) attributed autofluorescence and inhibition of cell division. Single-cell gel electrophoresis (comet assay) revealed UVA-induced single-strand DNA breaks. According to these results, UVA excitation radiation in fluorescence microscopy may damage cells. This has to be considered in vital cell microscopy, e.g., in calcium measurements.
Karsten Koenig, Tatiana B. Krasieva, Eckhard Bauer, Ursula Fiedler, Michael W. Berns, Bruce J. Tromberg, Karl-Otto Greulich, "Cell damage by UVA radiation of a mercury microscopy lamp probed by autofluorescence modifications, cloning assay, and comet assay," Journal of Biomedical Optics 1(2), (2 April 1996). https://doi.org/10.1117/12.233373
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