1 July 1996 Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations
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Rat lenses incubated in dimethyl sulfoxide (DMSO)–water binary mixtures of different compositions became turbid. A slight haziness developed up to 0.062 mole fraction of DMSO; at higher concentrations dense turbidity developed. Microscopic examination of the incubated rat lenses showed damage restricted to the epithelium and outer cortex at low DMSO concentrations; maximum damage occurred, in terms of fiber cell swelling, extracellular fluid (lake) formation and disintegration of epithelium, at 0.25 mole ratio of DMSO. Scanning electron microscopic observation showed that at high DMSO concentrations, especially at 0.25 mole fraction, the contents of the fiber cells were greatly damaged. Apparently a large part of the crystallins had coalesced around the cytoskeletal bodies. Polarized light-scattering intensity measurements and their analyses indicated that when dense opacities developed at high DMSO concentrations, the major contribution to the turbidity came from the optical anisotropy fluctuations. The change in the organization of the components within the fiber cells disturbs the balance between intrinsic birefringence and form birefringence necessary for transparency. Thus, the DMSO-caused opacification can be described as a good model for the involvement of optical anisotropy fluctuations in cataractogenesis.
Frederick A. Bettelheim, Frederick A. Bettelheim, A. Coolidge Churchill, A. Coolidge Churchill, W. Gerald Robison, W. Gerald Robison, J. Samuel Zigler, J. Samuel Zigler, } "Dimethyl sulfoxide cataract: a model for optical anisotropy fluctuations," Journal of Biomedical Optics 1(3), (1 July 1996). https://doi.org/10.1117/12.240660 . Submission:


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