1 March 2005 Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence
Author Affiliations +
J. of Biomedical Optics, 10(2), 024015 (2005). doi:10.1117/1.1886729
Abstract
Second-harmonic generation (SHG) and two-photon excitation fluorescence (TPEF) are relatively new and promising tools for the detailed imaging of biological samples and processes at the microscopic level. By exploiting these nonlinear phenomena phototoxicity and photobleaching effects on the specimens are reduced dramatically. The main target of this work was the development of a compact inexpensive and reliable experimental apparatus for nonlinear microscopy measurements. Femtosecond laser pulses were utilized for excitation. We achieved high-resolution imaging and mapping of Caenorhabditis elegans (C. elegans) neurons and muscular structures of the pharynx, at the microscopic level by performing SHG and TPEF measurements. By detecting nonlinear phenomena such as SHG and TPEF it is feasible to extract valuable information concerning the structure and the function of nematode neurons.
Giorgos Filippidis, C. Kouloumentas, G. Voglis, F. Zacharopoulou, Theodore G. Papazoglou, N. Tavernarakis, "Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence," Journal of Biomedical Optics 10(2), 024015 (1 March 2005). http://dx.doi.org/10.1117/1.1886729
JOURNAL ARTICLE
8 PAGES


SHARE
KEYWORDS
Second-harmonic generation

Neurons

Signal detection

Green fluorescent protein

Molecules

Luminescence

Microscopy

RELATED CONTENT

Applications of rigid and flexible GRIN-endoscopes
Proceedings of SPIE (February 15 2007)
Structure of poly(p-hydroxystyrene) film
Proceedings of SPIE (June 01 1991)
Aerosol classification study by lidar
Proceedings of SPIE (October 20 2015)

Back to Top