1 March 2005 Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra
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J. of Biomedical Optics, 10(2), 024016 (2005). doi:10.1117/1.1891370
Abstract
Spectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While two-photon excited autofluorescence is observed ubiquitously, the identities and distributions of endogenous fluorophores have not been completely characterized in most tissues. We develop an image-guided spectral analysis method to analyze the distribution of fluorophores in human skin from 3-D resolved two-photon images. We identify five factors that contribute to most of the luminescence signals from human skin. Luminescence species identified include tryptophan, NAD(P)H, melanin, and elastin, which are autofluorescent, and collagen that contributes to a second harmonic signal.
Lily Laiho Hsu, Serge Blaise Pelet, Thomas M. Hancewicz, Peter D. Kaplan, Peter T. C. So, "Two-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectra," Journal of Biomedical Optics 10(2), 024016 (1 March 2005). http://dx.doi.org/10.1117/1.1891370
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KEYWORDS
Skin

Luminescence

Tissues

Tissue optics

Spectroscopy

Photons

Collagen

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