1 May 2005 Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy
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Abstract
We demonstrated the feasibility of Raman spectroscopy for remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm–1 were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14–34 °C. The ratio between the integrated Raman intensities of two temperature dependent OH-vibrational regions (OH2/OH1) in the spectra of water showed high linear dependence on temperature both in pure water [0.0049(±1.2%)T+0.4522(±0.5%), R2=0.99, n=50, p<0.05], as well as in the rabbit aqueous humor [0.0036(±2.8%)T+0.4966(±0.6%), R2=0.98, n=162, p<0.05] with a high degree of reproducibility and sensitivity (~0.2–0.7 °C). Raman spectroscopy can be used for high resolution and remote monitoring of temperature in the aqueous humor under in vivo conditions.
© (2005) Society of Photo-Optical Instrumentation Engineers (SPIE)
Noel J.C. Bauer, Noel J.C. Bauer, Massoud Motamedi, Massoud Motamedi, Fred Hendrikse, Fred Hendrikse, James P. Wicksted, James P. Wicksted, } "Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy," Journal of Biomedical Optics 10(3), 031109 (1 May 2005). https://doi.org/10.1117/1.1911901 . Submission:
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