1 May 2005 Glucose determination in human aqueous humor with Raman spectroscopy
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Abstract
It has been suggested that spectroscopic analysis of the aqueous humor of the eye could be used to indirectly predict blood glucose levels in diabetics noninvasively. We have been investigating this potential using Raman spectroscopy in combination with partial least squares (PLS) analysis. We have determined that glucose at clinically relevant concentrations can be accurately predicted in human aqueous humor in vitro using a PLS model based on artificial aqueous humor. We have further determined that with proper instrument design, the light energy necessary to achieve clinically acceptable prediction of glucose does not damage the retinas of rabbits and can be delivered at powers below internationally acceptable safety limits. Herein we summarize our current results and address our strategies to improve instrument design.
© (2005) Society of Photo-Optical Instrumentation Engineers (SPIE)
James L. Lambert, James L. Lambert, Christine C. Pelletier, Christine C. Pelletier, Mark S. Borchert, Mark S. Borchert, } "Glucose determination in human aqueous humor with Raman spectroscopy," Journal of Biomedical Optics 10(3), 031110 (1 May 2005). https://doi.org/10.1117/1.1914843 . Submission:
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