1 May 2005 Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis
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J. of Biomedical Optics, 10(3), 034005 (2005). doi:10.1117/1.1924713
Abstract
Surface plasmon resonance (SPR) phase-shift interferometry (PSI) is a novel technique which combines SPR and modified Mach–Zehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5×10–7 refraction index change, a long-term phase stability of π/100 in 30 min, and a spatial phase resolution of π/300 with 100×100 µm2 detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray.
Shean-Jen Chen, Yuan-Deng Su, Feng-Ming Hsiu, Chia-Yuan Tsou, Yi-Kuang Chen, "Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis," Journal of Biomedical Optics 10(3), 034005 (1 May 2005). http://dx.doi.org/10.1117/1.1924713
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KEYWORDS
Imaging systems

Surface plasmons

Phase measurement

Reconstruction algorithms

Gold

Refractive index

Biological research

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