1 July 2005 Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging
Author Affiliations +
J. of Biomedical Optics, 10(4), 041207 (2005). doi:10.1117/1.2032458
Abstract
The ability to image and quantitate fluorescently labeled markers in vivo has generally been limited by autofluorescence of the tissue. Skin, in particular, has a strong autofluorescence signal, particularly when excited in the blue or green wavelengths. Fluorescence labels with emission wavelengths in the near-infrared are more amenable to deep-tissue imaging, because both scattering and autofluorescence are reduced as wavelengths are increased, but even in these spectral regions, autofluorescence can still limit sensitivity. Multispectral imaging (MSI), however, can remove the signal degradation caused by autofluorescence while adding enhanced multiplexing capabilities. While the availability of spectral "libraries" makes multispectral analysis routine for well-characterized samples, new software tools have been developed that greatly simplify the application of MSI to novel specimens.
James R. Mansfield, Kirk W. Gossage, Clifford C. Hoyt, Richard M. Levenson, "Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging," Journal of Biomedical Optics 10(4), 041207 (1 July 2005). http://dx.doi.org/10.1117/1.2032458
JOURNAL ARTICLE
9 PAGES


SHARE
KEYWORDS
Quantum dots

Luminescence

Multiplexing

Multispectral imaging

RGB color model

In vivo imaging

Skin

Back to Top