1 September 2005 Imaging of gene expression in living cells and tissues
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Abstract
It is possible to observe gene expression within single cells using a tetracycline inducible promoter for activation. Transcription can be observed by using a fluorescent fusion protein to bind nascent RNA. Ultimately, it is desirable to activate a reporter gene within a single cell with only photons. This is achieved by preparing a chemically altered transcription factor that is functionally unable to activate a reporter gene until it is exposed to photon excitation. We apply two-photon imaging to visualize tumor cells expressing a transgene and ultimately this approach will provide the means to activate a specific gene within a single cell within any tissue to ultimately observe its functional significance in situ.
© (2005) Society of Photo-Optical Instrumentation Engineers (SPIE)
Robert H. Singer, Robert H. Singer, David Lawrence, David Lawrence, Ben Ovryn, Ben Ovryn, John Condeelis, John Condeelis, } "Imaging of gene expression in living cells and tissues," Journal of Biomedical Optics 10(5), 051406 (1 September 2005). https://doi.org/10.1117/1.2103032 . Submission:
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