1 September 2005 Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
Author Affiliations +
Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2×2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2×2 fiber arrays.
© (2005) Society of Photo-Optical Instrumentation Engineers (SPIE)
Michael Gösch, Hans Blom, Sylvain Anderegg, Kerstin Korn, Per Thyberg, Mona Wells, Theo Lasser, Rudolf Rigler, Anders Magnusson, Sverker Hard, "Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements," Journal of Biomedical Optics 10(5), 054008 (1 September 2005). https://doi.org/10.1117/1.2080707 . Submission:

Back to Top