1 September 2005 Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements
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Abstract
Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2×2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2×2 fiber arrays.
© (2005) Society of Photo-Optical Instrumentation Engineers (SPIE)
Michael Gösch, Michael Gösch, Hans Blom, Hans Blom, Sylvain Anderegg, Sylvain Anderegg, Kerstin Korn, Kerstin Korn, Per Thyberg, Per Thyberg, Mona Wells, Mona Wells, Theo Lasser, Theo Lasser, Rudolf Rigler, Rudolf Rigler, Anders Magnusson, Anders Magnusson, Sverker Hard, Sverker Hard, } "Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements," Journal of Biomedical Optics 10(5), 054008 (1 September 2005). https://doi.org/10.1117/1.2080707 . Submission:
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