1 January 2006 Measurement of action spectra of light-activated processes
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J. of Biomedical Optics, 11(1), 014008 (2006). doi:10.1117/1.2161172
Abstract
We report on a new experimental technique suitable for measurement of light-activated processes, such as fluorophore transport. The usefulness of this technique is derived from its capacity to decouple the imaging and activation processes, allowing fluorescent imaging of fluorophore transport at a convenient activation wavelength. We demonstrate the efficiency of this new technique in determination of the action spectrum of the light mediated transport of rhodamine 123 into the parasitic protozoan Giardia duodenalis.
Justin Ross, Andrei V. Zvyagin, Norman Richard Heckenberg, Jacqui Upcroft, Peter Upcroft, Halina H. Rubinsztein-Dunlop, "Measurement of action spectra of light-activated processes," Journal of Biomedical Optics 11(1), 014008 (1 January 2006). http://dx.doi.org/10.1117/1.2161172
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KEYWORDS
Luminescence

Rhodamine

Imaging systems

Image processing

Biomedical optics

Bandpass filters

Confocal microscopy

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