1 January 2006 Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence
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Abstract
A scanning optical system for the detection of bacteria on meat surfaces based on fluorescence lifetime and intensity measurements is described. The system detects autofluorescent light emitted by naturally occurring fluorophores in bacteria. The technique only requires minimal sample preparation and handling, thus the chemical properties of the specimen are preserved. This work presents the preliminary results obtained from a time-resolved fluorescence imaging system for the characterization of a nonpathogenic gram-negative bacteria, Pseudomonas fluorescens. Initial results indicate that the combination of fluorescence lifetime and intensity measurements provides a means for characterizing biological media and for detecting microorganisms on surfaces.
© (2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Alain Bouchard, Julie Frechette, Marcia L. Vernon, Jean-François Cormier, Rene M. Beaulieu, Réal Vallée, Akier Assanta Mafu, "Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence," Journal of Biomedical Optics 11(1), 014011 (1 January 2006). https://doi.org/10.1117/1.2162166 . Submission:
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