Near-infrared spectroscopy (NIRS) can measure the product of the optical path length and the concentration change in oxygenated hemoglobin (ΔC′oxy), deoxygenated hemoglobin (ΔC′deoxy), and their sum (ΔC′total) in the human cerebral cortex, and it has been used for noninvasive investigation of human brain functions. We evaluate the within-subject reproducibility of the NIRS signals by repeated measurement of the sensorimotor cortex in healthy adults taken over a period of about 6 months using near-infrared (NIR) topography. The maximum signal amplitudes and the location of activation centers are compared between two sessions for each subject. The signal amplitudes vary between sessions and no consistent tendency in the changes is found among subjects. However, the distance between the activation centers identified in two sessions is relatively small, within 20 mm on average across subjects, which is comparable to the smallest distance between measurement positions in the NIR topography (21 mm). Moreover, within-subject comparisons of signal time courses show high correlation coefficients (<0.8) between the two sessions. This result, demonstrating a high within-subject reproducibility of the temporal information in NIRS signals, particularly contributes to the development of a new application of NIRS.