1 March 2006 Ability of laser fluorescence device associated with fluorescent dyes in detecting and quantifying early smooth surface caries lesions
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J. of Biomedical Optics, 11(2), 024007 (2006). doi:10.1117/1.2191046
Abstract
A laser fluorescence (LF) device is a portable tool, but it does not measure minor mineral changes. Our in vitro study aim is to propose the association of an LF with two fluorescent dyes and to evaluate the performance in detecting and quantifying early demineralization. Artificial caries lesions are created in 40 primary canine teeth using a demineralizing solution (pH=4.8) for 12, 24, 48, and 96 h. LF measurements are performed with DIAGNOdent after demineralization in these samples and in 20 sound primary teeth. Measurements with LF with 0.2-mM tetrakis(N-methylpyridyl)porphyrin (LF TMPyP) and with 4-mM protoporphyrin IX (LF PPIX) are made. The amount of calcium loss is determined by atomic emission spectrometry. A correlation between LF and LF with dyes and mineral loss and receiver operating characteristics analysis are performed, as well as comparisons of sensitivity, specificity, and accuracy values. Significant correlation is obtained with LF TMPyP and mineral loss of lesions demineralized for 24, 48, and 96 h. Better performance is achieved with LF TMPyP for all parameters than with LF alone. LF PPIX does not present good results. In conclusion, LF TMPyP provides good performance in detecting and quantifying very early enamel caries lesions.
Fausto Medeiros Mendes, Elisabeth de Oliveira, Dalva Lúcia Araújo de Faria, José Nicolau, "Ability of laser fluorescence device associated with fluorescent dyes in detecting and quantifying early smooth surface caries lesions," Journal of Biomedical Optics 11(2), 024007 (1 March 2006). http://dx.doi.org/10.1117/1.2191046
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KEYWORDS
Dental caries

Teeth

Calcium

Luminescence

Minerals

Laser dentistry

Natural surfaces

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