1 July 2006 Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis
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J. of Biomedical Optics, 11(2), 024011 (2006). doi:10.1117/1.2187013
Abstract
Caspase-2 is important for the mitochondrial apoptotic pathway, however, the mechanism by which caspase-2 executes apoptosis remains obscure. We carry out the first measurements of the dynamics of caspase-2 activation in a single living cell by a FRET (fluorescence resonance energy transfer) probe. Two FRET probes are constructed that each encoded a CRS (caspase-2 or caspase-3 recognition site) fused with a cyan fluorescent protein (CFP) and a red fluorescent protein (DsRed) (CFP-CRS-DsRed). Using these probes, we found that during cisplatin-induced apoptosis, caspase-2 activation occurred more slowly than did activation of caspase-3; additionally, caspase-2 activation was initiated much earlier than that of caspase-3.
Juqiang Lin, Zhihong Zhang, Jie Yang, Shaoqun Zeng, Bifeng Liu, Qingming Luo, "Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis," Journal of Biomedical Optics 11(2), 024011 (1 July 2006). http://dx.doi.org/10.1117/1.2187013
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KEYWORDS
Fluorescence resonance energy transfer

Cell death

Proteins

Fluorescent proteins

In vitro testing

Luminescence

Imaging systems

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