1 May 2006 Adaptive virus detection using filament-coupled antibodie
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Abstract
We recently reported the development of a filament-antibody recognition assay (FARA), in which the presence of virions in solution initiates the formation of enzyme-linked immunosorbent assay (ELISA)-like antibody complexes. The unique features of this assay are that processing is achieved by motion of a filament and that, in the presence of a virus, antibody-virus complexes are coupled to the filament at known locations. In this work, we combine the unique features of this assay with a 638-nm laser-based optical detector to enable adaptive control of virus detection. Integration of on-line fluorescence detection yields approximately a five-fold increase in signal-to-noise ratio (SNR) compared to the fluorescence detection method reported previously. A one-minute incubation with an M13K07 test virus is required to detect 1010 virions/ml, and 40 min was required to detect 108 virions/ml. In tests of the components of an adaptive strategy, a 30-min virus (3.3×1010 virions/ml) incubation time, followed by repositioning the filament-captured virus either within the detecting antibody chamber, (20µg/ml) or within the virus chamber, found an increase in signal roughly proportional to the cumulative residence times in these chambers. Furthermore, cumulative fluorescence signals observed for a filament-captured virus after repeated positioning of the filament within the virus chamber are similar to those observed for a single long incubation time. The unique features of the FARA-like design combined with online optical detection to direct subsequent bioprocessing steps provides new flexibility for developing adaptive molecular recognition assays.
© (2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Gregory P. Stone, Kelvin S. Lin, Frederick R. Haselton, "Adaptive virus detection using filament-coupled antibodie," Journal of Biomedical Optics 11(3), 034012 (1 May 2006). https://doi.org/10.1117/1.2209907 . Submission:
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