1 May 2006 In-vivo imaging of tumor associated urokinase-type plasminogen activator activity
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J. of Biomedical Optics, 11(3), 034013 (2006). doi:10.1117/1.2204029
Abstract
The ability to image tumor associated protease in vivo has biological and clinical implications. In the present study, we describe the development and validation of a urokinase-type plasminogen activator (uPA) sensitive fluorescence imaging probe. The activation of our probe is highly specific to uPA in both enzymatic and cellular-based assays. In two distinct in-vivo tumor models (human colon adenocarcinoma HT-29 and human fibrosarcoma HT-1080), the observed fluorescence changes correlate well with tumor associated uPA activity. The signal intensities of the tumors are about three-fold higher in animals with probe injections. Our results suggest a direct detection method for uPA activity in vivo and the approach can be used for monitoring tumor growth and development.
Jong-Kai Hsiao, Benedict Law, Ralph Weissleder, Ching Hsuan Tung, "In-vivo imaging of tumor associated urokinase-type plasminogen activator activity," Journal of Biomedical Optics 11(3), 034013 (1 May 2006). https://doi.org/10.1117/1.2204029
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