1 May 2006 Novel nontoxic mitochondrial probe for confocal fluorescence microscopy
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Abstract
We propose a 2,5-Bis[1-(4-N-methylpyridinium)ethen-2-yl)]-N-methylpyrrole ditriflate (PEPEP) as a novel nontoxic, nonpotentiometric mitochondrial probe for confocal fluorescence microscopy. PEPEP is a representative chromophore of a large family of heterocyclic fluorescent dyes that show fluorescence emission in aqueous media and great DNA affinity. We check its cytotoxicity and intracellular localization in mammalian and yeast cell cultures. We demonstrate that PEPEP is a very efficient dye for fluorescence confocal microscopy and a valuable alternative to the most frequently used mitochondrial stains.
© (2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Silvia Versari, Silvia Versari, Anna Maria Villa, Anna Maria Villa, Alessandro Villa, Alessandro Villa, Silvia M. Doglia, Silvia M. Doglia, Giorgio A. Pagani, Giorgio A. Pagani, Silvia Bradamante, Silvia Bradamante, } "Novel nontoxic mitochondrial probe for confocal fluorescence microscopy," Journal of Biomedical Optics 11(3), 034014 (1 May 2006). https://doi.org/10.1117/1.2206173 . Submission:
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