1 May 2006 Prediction of heat-induced collagen shrinkage by use of second harmonic generation microscopy
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Abstract
Collagen shrinkage associated with denaturation from thermal treatment has a number of important clinical applications. However, individualized treatment is hindered by the lack of reliable noninvasive methods to monitor the process of collagen denaturation. We investigate the serial changes of collagen denaturation from thermal treatment of rat tail tendons at 58 °C by use of second harmonic generation (SHG) microscopy. We find that rat tail tendon shrinks progressively from 0 to 9 min of thermal treatment, and remains unchanged in length upon further thermal treatment. The SHG intensity also decreases from 0 to 9 min of thermal treatment and becomes barely detectable from further thermal treatment. Collagen shrinkage and the SHG intensity are well correlated in a linear model. In addition, SHG imaging reveals a tiger-tail-like pattern of collagen denaturation. The bands of denatured collagen progressively widen from increased thermal treatment and completely replace the adjacent bands of normal collagen after 9 min of thermal treatment. Our results show that collagen denaturation in rat tail tendon from thermal treatment is inhomogeneous, and that SHG intensity can be used to predict the degree of thermally induced collagen shrinkage. With additional development, this approach has the potential to be used in biomedical applications.
© (2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Sung-Jan Lin, Sung-Jan Lin, Wen Lo, Wen Lo, Hsin-Yuan Tan, Hsin-Yuan Tan, Jung-Yi Chan, Jung-Yi Chan, Wei-Liang Chen, Wei-Liang Chen, Shiou-Han Wang, Shiou-Han Wang, Yen Sun, Yen Sun, Wei-Chou Lin, Wei-Chou Lin, Jau-Shiuh Chen, Jau-Shiuh Chen, Chih-Jung Hsu, Chih-Jung Hsu, Jen-Wei Tiju, Jen-Wei Tiju, Hsin-Su Yu, Hsin-Su Yu, Shiou-Hwa Jee, Shiou-Hwa Jee, Chen-Yuan Dong, Chen-Yuan Dong, } "Prediction of heat-induced collagen shrinkage by use of second harmonic generation microscopy," Journal of Biomedical Optics 11(3), 034020 (1 May 2006). https://doi.org/10.1117/1.2209959 . Submission:
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